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Visualizing Changes in Protein Synthesis | FUNCAT Method
WEBWith FUNCAT the researchers were able to detect changes in protein synthesis, analyze the fate of proteins, and visualize the dynamics of newly synthesized membrane proteins with single particle tracking and quantum dots.
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In situ visualization and dynamics of newly synthesized proteins …
WEBJun 13, 2010 · Nature Neuroscience - Dieterich et al. describe a methodology to label all newly synthesized neuronal proteins in situ. This method, which they name FUNCAT, relies on the inclusion of...
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Cell Press: STAR Protocols
WEBDec 14, 2023 · The fluorescent non-canonical amino acid tagging (FUNCAT) technique has been used to visualize newly synthesized proteins in cell lines and tissues. Here, we present a protocol for measuring protein synthesis in specific cell types in the mouse brain using in vivo FUNCAT.
Direct visualization of newly synthesized target proteins in situ
WEBMar 16, 2015 · In FUNCAT, azidohomoalanine (AHA) is taken up by cells and loaded onto methionine tRNAs. During translation, AHA is incorporated into newly synthesized proteins.
The FunCat, a functional annotation scheme for systematic ...
WEBOct 14, 2004 · In this paper, we present the Functional Catalogue (FunCat), a hierarchically structured, organism-independent, flexible and scalable controlled classification system enabling the functional description of proteins from any organism.
Main functional categories of the FunCat | Download Table
WEBIn this paper, we present the Functional Catalogue (FunCat), a hierarchically structured, organism-independent, flexible and scalable controlled classification system enabling the functional...
The FunCat, a functional annotation scheme for systematic ...
WEBFeb 1, 2004 · FunCat has been applied for the manual annotation of prokaryotes, fungi, plants and animals. We describe how FunCat is implemented as a highly efficient and robust tool for the manual and...
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