Most researchers know they need a pure protein. Few talk about how genuinely difficult it is to get one. A cell lysate contains thousands of proteins competing for the same space. Your target is in ...
Tedious, time-consuming, exasperating: Those are perhaps the three words that researchers use most commonly to describe protein purification. Proteins, and their little sisters the peptides, are among ...
Protein purification is both an art and a science, requiring the right combination of techniques, equipment, and molecular insights. From hydrophobic interaction chromatography (HIC) to reversed phase ...
Researchers at Queen’s University in Kingston, Ontario, Canada, are developing an alternative to nickel-nitrilotriacetic acid (Ni-NTA) to purify recombinant proteins. Because it uses common ...
Proteins play a key role in the life sciences - from basic research and biotechnological applications to the development and manufacturing of pharmaceuticals. Scientists at the Technical University of ...
The team has further developed the method of chromatography. For this purpose, they have developed a light-sensitive molecular appendix that serves as an anchor for the target protein. It binds the ...
For the novice protein purifier, finding a place to start might seem overwhelming, but fortunately the process can be approached systematically. Andrew Mitchell, technical consultant for Amersham ...
Protein and antibody purification underpins the creation of safe, effective biopharmaceuticals. From antibody capture using ...
Membrane proteins are the gateway to cells and are involved in many critical functions. Each membrane protein is unique, and their complexity makes it difficult to fit into a standardized expression ...