DNA-based methods dominate molecular cancer diagnostics but struggle to detect gene fusions and assess splice site consequences. RNA sequencing enables sensitive fusion detection and direct assessment ...

Accurate assembly of rRNA-depleted (total) RNA sequencing (RNA-seq) remains challenging because existing methods often conflate incomplete, nascent RNA with fully processed mature isoforms, leading to ...

Alternative polyadenylation (APA) and alternative splicing (AS) are essential post-transcriptional mechanisms that enhance transcriptome diversity and regulate gene expression across various ...

Researchers from Children's Hospital of Philadelphia (CHOP) developed a new RNA sequencing strategy that can reveal how genetic variants disrupt gene function and improve the diagnosis of rare ...

While bulk sequencing quickly became the preferred method, scRNA-seq has increased in popularity over the last decade. In 2015, just 6 % of RNA-seq papers used single-cell methodologies. By 2025, the ...

A new single-cell technology is giving scientists their clearest view yet of immune cell behavior—capturing not just genetic intent, but real-time activity. By measuring RNA and proteins ...

Morgan Hiebert (left) studies drug-resistant tuberculosis within the National Reference Centre for Mycobacteriology (Winnipeg, Manitoba) led by Hafid Soualhine, situated in the National Microbiology ...

Evaluation of scCNV inference methods using a clinical small cell lung cancer (SCLC) dataset. (A) Sensitivity and specificity of the four scCNV inference methods applied to primary and relapse SCLC ...

A comprehensive review article titled “Bioinformatics perspectives on transcriptomics: A comprehensive review of bulk and single-cell RNA sequencing analyses,” published in Quantitative Biology, ...

Understand the stages of a standard RNA-seq bioinformatics workflow, including the role of QC, alignment, gene expression quantification, and specialized application analyses Identify key QC metrics ...

Inside every cell, thousands of molecular signals collide, overlap, and compensate, obscuring the true drivers of gene expression. Scientists have now developed a way to silence that cellular noise, ...

Bacteria aren’t just mindless microbes. Research by scientists at the Hebrew University of Jerusalem has revealed that single bacterial cells can carry a “memory” of their past environments—passing it ...